Application

Hydrocortisone is used for the following applications:Used in myogenic induction and identification Used in long-term stromal cultures and methylcellulose cultures (one of the components in the culture medium)Used in adipogenic differentiation (one of the components in adipogenic medium)Used in cell culture: The human mammary epithelial cell MCF-10A cells were maintained in DMEM/F12 and supplemented with hydrocortisone along with other componentsThe SUM149 cell line was established from a primary IBC and grown in FBS supplemented with Ham′s F12 medium containing insulin and hydrocortisoneUsed in differentiation assays of melanoma spheroid cells

Biochem/physiol Actions

Primary glucocorticoid secreted by the adrenal cortex. It has three times the anti-inflammatory potency of corticosterone but much lower Na²⁺ retention potency.

General description

Hydrocortisone for use in epithelial and endothelial adherent cell culture applications.

Physical form

powder-RT; stock-frozen in working aliquots, avoid repeated freeze/thaw

Reconstitution

To prepare 50 µg/mL stock solution; add 1 mL absolute ethanol, gently swirl to dissolve, add 19 mL sterile medium while mixing.